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Base de datos :	IBECS
Búsqueda :	"1139-6709" [ISSN]
Referencias encontradas :	814 [refinar]
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Id:	2
Autor:	Ortega, Leonel Maximiliano; Moure, María Candela; González, Esteban Manuel; Alconada, Teresa María.
Título:	Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of Fusarium graminearum
Fuente:	Int. microbiol;22(2):289-296, jun. 2019. graf, tab.
Idioma:	en.
doi:	10.1007/s10123-018-00048-y.
Resumen:	Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grain infection with Fusarium graminearum produce changes in the glutenin content that affect the baking properties. This work analyzes the high-molecular-weight glutenin changes from wheat flour with different degrees of F. graminearum infection at field, since these proteins are determinant for the quality properties of flour. Wheat cultivars-on field trials-infected with F. graminearum isolates of diverse aggressiveness showed severity values between 9.1 and 42.58% and thousand kernel weight values between 28.12 and 32.33 g. Negative correlations between severity and protein content and positive correlations between yield and protein content were observed, employing reversed-phase high-performance liquid chromatography and polyacrylamide gel electrophoresis. Furthermore, the protein signal changes were in agreement for both methodological approaches. Also, the degree of disease observed and the protein changes on infected wheat cultivars varied in relation with the aggressiveness of the isolate responsible for the infection. The principal component analysis showed a close arrangement among protein values obtained by HPLC. For each cultivar, two principal components were obtained, which explained 80.85%, 88.48%, and 93.33% of the total variance (cultivars Sy200, AGP Fast, and Klein Tigre respectively). To our knowledge, the approaches employed for the analysis of protein changes according to the degree of disease, as well as the thorough statistical analysis, are novel for the study of Fusarium Head Blight No disponible
Descriptores:	Fusarium/metabolismo glútenes/análisis enfermedades de las plantas/microbiología proteínas de plantas/análisis Triticum/microbiología
	-Triticum/química Fusarium/crecimiento & desarrollo harina/análisis
Responsable:	BNCS

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Id:	232307
Autor:	Eski, Ardahan; Erdoğan, Pervin; Demirbağ, Zihni; Demir, İsmail.
Título:	Isolation and identification of bacteria from the invasive pest Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) and evaluation of their biocontrol potential
Fuente:	Int. microbiol;27(2):631-643, Abr. 2024. graf.
Idioma:	en.
doi:	10.1007/s10123-023-00418-1.
Resumen:	As an alternative to chemical insecticides, gut bacteria of insects could be used to control insect pests. In this study, bacteria associated with Tuta absoluta, an invasive species that has developed resistance to chemical insecticides, were isolated, and their potential for pest control was investigated. We isolated 13 bacteria from larvae of the pest and identified the isolates on the basis of their morphological, physiological, biochemical, and molecular characteristics as Bacillus thuringiensis (Ta1-8), Staphylococcus petrasii (Ta9), Citrobacter freundii (Ta10), Chishuiella changwenlii (Ta11), Enterococcus casseliflavus (Ta12), and Pseudomonas tremae (Ta13). A laboratory screening test at 109 cfu/ml showed that B. thuringiensis (Bt) isolates caused more than 90% mortality after 3 days. Among the isolates, Bt-Ta1 showed the highest mortality in a short time. The LC50 and LC90 values for Bt-Ta1 were estimated to be 1.2 × 106 and 2 × 109 cfu/ml, respectively. Detailed characterization of Bt-Ta1 revealed that it is one of the serotypes effective on lepidopterans and contains the genes cry1Aa, cry2Aa, and vip3Aa, which encode lepidopteran toxic proteins. Bt-Ta1 isolate has been shown to have the potential to be used in the integrated management of Tuta absoluta. (AU)
Descriptores:	insecticidas agroquímicos Bacteria insectos plagas agrícolas
Tipo de Publicación:	estudio observacional
Responsable:	ES15.1 - BNCS

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Id:	232306
Autor:	Lenchi, Nesrine; El Houda Ahmedi, Wissam Nour; Llirós, Marc.
Título:	Simultaneous removal of crude oil and heavy metals by highly adapted bacterial strain Cutibacterium sp. NL2 isolated from Algerian oilfield
Fuente:	Int. microbiol;27(2):615-630, Abr. 2024. graf.
Idioma:	en.
doi:	10.1007/s10123-023-00419-0.
Resumen:	Investigating the ability of bacteria to simultaneously enhance hydrocarbon removal and reduce heavy metals' toxicity is necessary to design more effective bioremediation strategies. A bacterium (NL2 strain) isolated from an Algerian oilfield was cultivated on crude oil as sole carbon and energy sources. Molecular analyses of the 16S rRNA gene sequence placed the strain within the Cutibacterium genera. This isolate was able to tolerate up to 60% of crude oil as sole carbon source. Chemical analyses (GC-MS) evidenced that strain NL2 was able to degrade 92.22% of crude oil (at optimal growing conditions: pH 10, 44 ºC, 50 g L−1 NaCl, and 20% of crude oil (v/v) as sole carbon source) in only 7 days. NL2 isolate was also able to produce biosurfactants with reduction of surface tension of growing media (29.4 mN m−1). On the other hand, NL2 strain was able to tolerate high lead (Pb) and copper (Cu) concentrations (up to 60 mM). In fact, NL2 cultivated in the presence of 20% of crude oil, and 0.48 mM of Pb was able to reduce Pb concentration by a 41.36%. In turn, when cultivated on high Pb concentration (15 mM), the strain was able to remove 35.19% of it and 86.25% of crude oil, both in a time frame of 7 days. Our findings suggest that Cutibacterium strain NL2 is able to efficiently use and remove a wide range of crude oil substrates in presence of high Pb concentration. Accordingly, NL2 strain is of extreme interest from a biotechnological standpoint. (AU)
Descriptores:	Bacteria hidrocarburos toxicidad metales pesados biodegradación ambiental industria del petróleo y gas
Tipo de Publicación:	informes de casos
Responsable:	ES15.1 - BNCS

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Id:	232305
Autor:	A. Mohite, Jyoti; S. Manvi, Shubha; Pardhi, Kajal; A. Bahulikar, Rahul; Deshpande, Shrinidhi; Patange, Sanjana; Joshi, Mansi; Kulkarni, Sharvari; C. Rahalkar, Monali.
Título:	Diverse type I and type II methanotrophs cultivated from an Indian freshwater wetland habitat
Fuente:	Int. microbiol;27(2):607-614, Abr. 2024. ilus.
Idioma:	en.
doi:	10.1007/s10123-023-00415-4.
Resumen:	Wetlands are the main natural sources of methane emissions, which make up a significant portion of greenhouse gas emissions. Such wetland patches serve as rich habitats for aerobic methanotrophs. Limited knowledge of methanotrophs from tropical wetlands widens the scope of study from these habitats. In the present study, a freshwater wetland in a tropical region in India was sampled and serially diluted to obtain methanotrophs in culture. This was followed by the isolation of methanotrophs on agarose-containing plates, incubated under methane: air atmosphere. Methanotrophs are difficult to cultivate, and very few cultures of methanotrophs are available from tropical wetlands. Our current study reports the cultivation of a diverse community of methanotrophs from six genera, namely, Methylomonas, Methylococcus, Methylomagnum, Methylocucumis (type I methanotrophs) along with Methylocystis, Methylosinus (type II methanotrophs). A high abundance of methanotrophs (106û1010 methanotrophs/g fresh weight) was observed in the samples. A Methylococcus strain could represent a putative novel species that was also isolated. Cultures of Methylomagnum and Methylocucumis, two newly described type I methanotrophs exclusively found in rice fields, were obtained. A large number of Methylomonas koyamae strains were cultured. Our study is pioneering in the documentation of culturable methanotrophs from a typical tropical wetland patch. The isolated methanotrophs can act as models for studying methanotroph-based methane mitigation from wetland habitats and can be used for various mitigation and valorization applications. (AU)
Descriptores:	humedales metano efecto invernadero gases ecosistema agua dulce
Tipo de Publicación:	estudio observacional
Responsable:	ES15.1 - BNCS

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Id:	232304
Autor:	Müller, Marius; Wiencierz, Andrea; Gehringer, Christian; Muigg, Veronika; Bassetti, Stefano; Siegemund, Martin; Hinic, Vladimira; Tschudin Sutter, Sarah; Egli, Adrian.
Título:	Factors associated with non-carbapenemase mediated carbapenem resistance of Gram-negative bacteria: a retrospective case-control study
Fuente:	Int. microbiol;27(2):597-606, Abr. 2024. graf.
Idioma:	en.
doi:	10.1007/s10123-023-00405-6.
Resumen:	Infections with carbapenemase-producing Gram-negative bacteria are related to increased morbidity and mortality, yet little is known regarding infections caused by non-beta-lactamase mediated carbapenem-resistant bacteria. Our objective was to identify risk factors for, and the clinical impact of infections caused by carbapenem-resistant carbapenemase-negative Enterobacterales and Pseudomonas aeruginosa. This retrospective matched case-control study was performed at the University Hospital of Basel, Switzerland, in 2016. We focused on other resistance mechanisms by excluding laboratory-confirmed carbapenemase-positive cases. Carbapenem resistance was set as the primary endpoint, and important risk factors were investigated by conditional logistic regression. The clinical impact of carbapenem resistance was estimated using regression models containing the resistance indicator as explanatory factor and adjusting for potential confounders. Seventy-five cases of infections with carbapenem-resistant, carbapenemase-negative bacteria were identified and matched with 75 controls with carbapenem-susceptible infections. The matched data set was well-balanced regarding age, gender, and comorbidity. Duration of prior carbapenem treatment (OR 1.15, [1.01, 1.31]) correlated with resistance to carbapenems. Our study showed that patients with carbapenem-resistant bacteria stayed 1.59 times (CI [0.81, 3.14]) longer in an ICU. The analyzed dataset did not provide evidence for strong clinical implications of resistance to carbapenems or increased mortality. The duration of prior carbapenem treatment seems to be a strong risk factor for the development of carbapenem resistance. The higher risk for a longer ICU stay could be a consequence of a carbapenem resistance. In contrast to carbapenemase-producers, the clinical impact of carbapenamase-negative, carbapenem-resistant strains may be limited... (AU)
Descriptores:	infecciones por bacterias gramnegativas morbilidad mortalidad INFECCIONES beta-lactamasas
Límites:	seres humanos adulto
Tipo de Publicación:	estudio observacional
Responsable:	ES15.1 - BNCS

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Id:	232303
Autor:	B. Khatape, Anil; Rangaswamy, Vidhya; G. Dastager, Syed.
Título:	Strain improvement for enhanced erythritol production by Moniliella pollinis Mutant-58 using jaggery as a cost-effective substrate
Fuente:	Int. microbiol;27(2):581-596, Abr. 2024. graf.
Idioma:	en.
doi:	10.1007/s10123-023-00411-8.
Resumen:	Erythritol has been produced by various microorganisms including Yarrowia, Moniliella, Aureobasidium, and Candida strains. Due to its relatively high price, erythritol sweetener is used lesser than other polyols despite having many advantages. Therefore, in this study, Moniliella pollinis strain was improved for erythritol production by chemical mutagenesis and subsequently screening for cost-effective carbon sources for the enhanced erythritol yield. M. pollinis was subjected to N-methyl N-nitro N-nitroso guanidine (NTG), ethyl methyl sulfonate (EMS), and UV mutagenesis for improved erythritol production. The fmutant strains were evaluated for enhanced erythritol production medium optimization by using different carbon substrates at the shake flask level. To enhance the production of erythritol and statistical media, optimization was carried out using a central composite design (CCD). Among 198 isolated mutants, Mutant-58 strain generated by EMS mutagenesis was selected for further assessment. The Mutant-58 strain showed significant morphological changes as compared to the parent strain. Furthermore, statistically optimized media composition resulted in the higher production of erythritol (91.2 ± 3.4 g/L) with a yield of 40.7 ± 3.4 % in shake flask experiments. The optimized medium composition for erythritol production constitutes (g/L) 225 jaggery, 4.4 yeast extract (YE), 4.4 KH2PO4, 0.31 MgSO4, and pH 5.5. The present study demonstrated strain improvement, media, and process optimization resulting in a 30% increase in the erythritol production in the Mutant-58 as compared to the parent strain. This is also the first instance where jaggery has been used as a cost-effective carbon source alternative to glucose for industrial-scale erythritol production. (AU)
Descriptores:	eritritol microorganismos acuáticos Yarrowia AUREOBASIDIUM Candida edulcorantes
Tipo de Publicación:	estudio comparativo
Responsable:	ES15.1 - BNCS

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Id:	232302
Autor:	Li, Siming; Young, Tim; Archer, Stephen; Lee, Kevin; C. Alfaro, Andrea.
Título:	Gut microbiome resilience of green-lipped mussels, Perna canaliculus, to starvation
Fuente:	Int. microbiol;27(2):571-580, Abr. 2024. graf.
Idioma:	en.
doi:	10.1007/s10123-023-00397-3.
Resumen:	Host gut microbiomes play an important role in animal health and resilience to conditions, such as malnutrition and starvation. These host-microbiome relationships are poorly understood in the marine mussel Perna canaliculus, which experiences significant variations in food quantity and quality in coastal areas. Prolonged starvation may be a contributory factor towards incidences of mass mortalities in farmed mussel populations, resulting in highly variable production costs and unreliable market supplies. Here, we examine the gut microbiota of P. canaliculus in response to starvation and subsequent re-feeding using high-throughput amplicon sequencing of the 16S rRNA gene. Mussels showed no change in bacterial species richness when subjected to a 14-day starvation, followed by re-feeding/recovery. However, beta bacteria diversity revealed significant shifts (PERMANOVA p-value < 0.001) in community structure in the starvation group and no differences in the subsequent recovery group (compared to the control group) once they were re-fed, highlighting their recovery capability and resilience. Phylum-level community profiles revealed an elevation in dominance of Proteobacteria (ANCOM-BC p-value <0.001) and Bacteroidota (ANCOM-BC p-value = 0.04) and lower relative abundance of Cyanobacteria (ANCOM-BC p-value = 0.01) in the starvation group compared to control and recovery groups. The most abundant genus-level shifts revealed relative increases of the heterotroph Halioglobus (p-value < 0.05) and lowered abundances of the autotroph Synechococcus CC9902 in the starvation group. Furthermore, a SparCC correlation network identified co-occurrence of a cluster of genera with elevated relative abundance in the starved mussels that were positively correlated with Synechococcus CC9902... (AU)
Descriptores:	microbiota intestinal veterinaria desnutrición hambre alimentos/clasificación inanición
Límites:	animales
Tipo de Publicación:	estudio observacional
Responsable:	ES15.1 - BNCS

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Id:	232301
Autor:	Gherbawy, Youssuf Ahmed; Aboelkassem Thabet, Maha; Sultan, Serageldeen.
Título:	Genetic and morphological characterization of a new genotype of nervous necrosis virus circulating among Nile tilapia in the south of Egypt
Fuente:	Int. microbiol;27(2):559-569, Abr. 2024. ilus.
Idioma:	en.
doi:	10.1007/s10123-023-00406-5.
Resumen:	Nervous necrosis virus (NNV) is the causative agent of viral nervous necrosis in freshwater and marine fishes. In this study, NNV circulating among wild and farmed Nile tilapia (Oreochromis niloticus) was genetically and morphologically characterized using reverse transcription polymerase chain reaction (RT-PCR), sequencing analysis, and transmission electron microscopy (TEM). Brain, eye, and other organ (spleen, kidney, heart, and liver) specimens were collected from 87 wild (66) and farmed (21) Nile tilapia fish during their adult or juvenile stage at different localities in Qena and Sohag governorates in southern Egypt. Among them, 57/87 fish showed suspected NNV clinical signs, and 30/87 were healthy. The results revealed that NNV was detected in 66 out of 87 fish (58.62% in the wild and 17.24% in farmed Nile tilapia by RT-PCR), and the prevalence was higher among diseased (55.17%) than in healthy (20.69%) fish. NNV was detected in the brain, eye, and other organs. Using TEM, virion size variations based on the infected organs were observed. Nucleotide sequence similarity indicated that NNVs had a divergence of 75% from other fish nodaviruses sequenced in Egypt and worldwide. Phylogenetic analysis distinguished them from other NNV genotypes, revealing the emergence of a new NNV genotype in southern Egypt. In conclusion, NNV is circulating among diseased and healthy Nile tilapia, and a new NNV genotype has emerged in southern Egypt. (AU)
Descriptores:	necrosis peces agua dulce genética ARN polimerasas dirigidas por ADN microscopía
Límites:	animales
Tipo de Publicación:	estudio observacional veterinario
Responsable:	ES15.1 - BNCS

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Id:	232300
Autor:	Houida, Sofia; Yakkou, Lamia; Kaya, Leyla Okyay; Bilen, Serdar; Raouane, Mohamed; El Harti, Abdellatif; Amghar, Souad.
Título:	Plant growth-promoting bacteria isolated from earthworms enhance spinach growth and its phytoremediation potential in metal-contaminated soils / Las bacterias promotoras del crecimiento vegetal aisladas de lombrices de tierra mejoran el crecimiento de las espinacas y su potencial de fitorremediación en suelos contaminados con metales
Fuente:	Int. microbiol;27(2):545-558, Abr. 2024. graf.
Idioma:	en.
doi:	10.1007/s10123-023-00402-9.
Resumen:	The aim of this study was to evaluate the impact of metal-tolerant plant growth-promoting bacteria (PGPB) isolated from the chloragogenous tissue of Aporrectodea molleri, which represents a unique habitat. Our objectives were to investigate their effects on the growth of Spinacia oleracea under heavy metal stress and assess their potential for enhancing phytoremediation capabilities. The experiment was conducted in an alkaline soil contaminated with 7 mg kg-1 of cadmium, 100 mg kg-1 of nickel, 150 mg kg-1 of copper, 300 mg kg-1 of Zinc, and mg kg-1 of 600 Manganese. The results showed that heavy metal stress considerably diminished root (42.8%) and shoot length (60.1%), biomass (80%), chlorophyll content (41%), soil alkaline (45%), and acid (51%) phosphatases (42%) and urease (42%). However, soil inoculation with bacterial isolates remarkably improved plant growth. Soil bioaugmentation increased spinach growth (up to 74.5% for root length, up to 106.3% for shoot length, and up to 5.5 folds for fresh biomass) while significantly increasing soil enzyme activity and NPK content. Multivariate data analysis indicated that soil inoculation with Bacillus circulans TC7 promoted plant growth while limiting metal bioaccumulation, whereas Pseudomonas sp. TC33 and Bacillus subtilis TC34 increased metal bioaccumulation in spinach tissues while minimizing their toxicity. Our study confirms that earthworms are a reservoir of multi-beneficial bacteria that can effectively improve phytoremediation efficiency and mitigate the toxic effects of heavy metals on plant growth. Further studies are needed to investigate the long-term effects and feasibility of using these isolates as a consortium in field applications.(AU)
Descriptores:	Oligochaeta contaminantes del suelo/análisis contaminantes del suelo/toxicidad Bacteria metales pesados/análisis
	-microbiología biodegradación ambiental
Límites:	seres humanos
Responsable:	ES15.1 - BNCS

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Id:	232299
Autor:	Li, Bai-Lin; Chen, Jia-Yan; Hu, Juan-Juan; Fan, Yu-Wen; Ao, Zhuo-Yi; Zhang, Wei-Jie; Lian, Xin; Liang, Hui-Jun; Li, Qian-Ran; Guan, Xiao-Xian.
Título:	Three stilbenes from pigeon pea with promising anti-methicillin-resistant Staphylococcus aureus biofilm formation activity / Tres estilbenos del gandul con prometedora actividad de formación de biopelículas de Staphylococcus aureus resistente a la meticilina
Fuente:	Int. microbiol;27(2):535-544, Abr. 2024. ilus.
Idioma:	en.
doi:	10.1007/s10123-023-00413-6.
Descriptores:	estilbenos antibacterianos/farmacología anticuerpos/farmacología biopelículas Staphylococcus aureus resistente a meticilina pruebas de sensibilidad microbiana
Límites:	seres humanos
Responsable:	ES15.1 - BNCS

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