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Búsqueda : "1139-6709" [ISSN]
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Id: 2
Autor: Ortega, Leonel Maximiliano; Moure, María Candela; González, Esteban Manuel; Alconada, Teresa María.
Título: Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of Fusarium graminearum
Fuente: Int. microbiol;22(2):289-296, jun. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-018-00048-y.
Resumen: Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grain infection with Fusarium graminearum produce changes in the glutenin content that affect the baking properties. This work analyzes the high-molecular-weight glutenin changes from wheat flour with different degrees of F. graminearum infection at field, since these proteins are determinant for the quality properties of flour. Wheat cultivars-on field trials-infected with F. graminearum isolates of diverse aggressiveness showed severity values between 9.1 and 42.58% and thousand kernel weight values between 28.12 and 32.33 g. Negative correlations between severity and protein content and positive correlations between yield and protein content were observed, employing reversed-phase high-performance liquid chromatography and polyacrylamide gel electrophoresis. Furthermore, the protein signal changes were in agreement for both methodological approaches. Also, the degree of disease observed and the protein changes on infected wheat cultivars varied in relation with the aggressiveness of the isolate responsible for the infection. The principal component analysis showed a close arrangement among protein values obtained by HPLC. For each cultivar, two principal components were obtained, which explained 80.85%, 88.48%, and 93.33% of the total variance (cultivars Sy200, AGP Fast, and Klein Tigre respectively). To our knowledge, the approaches employed for the analysis of protein changes according to the degree of disease, as well as the thorough statistical analysis, are novel for the study of Fusarium Head Blight

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Descriptores: Fusarium/metabolismo
glútenes/análisis
enfermedades de las plantas/microbiología
proteínas de plantas/análisis
Triticum/microbiología
-Triticum/química
Fusarium/crecimiento & desarrollo
harina/análisis
Responsable: BNCS


  2 / 790 IBECS  
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Id: 230264
Autor: Amin, Aaima; Noureen, Ramisha; Iftikhar, Ayesha; Hussain, Annam; Alonazi, Wadi B; Zeeshan Raza, Hafiz Muhammad; Ferheen, Ifra; Ibrahim, Muhammad.
Título: Uropathogenic bacteria and deductive genomics towards antimicrobial resistance, virulence, and potential drug targets / Bacterias uropatógenas y genómica deductiva hacia la resistencia, virulencia y posibles objetivos farmacológicos a los antimicrobianos
Fuente: Int. microbiol;27(1):325-335, Feb. 2024. mapas.
Idioma: en.
doi: 10.1007/s10123-023-00416-3.
Resumen: Urinary tract infections (UTIs) are among the most prevalent bacterial infections affecting people in inpatient and outpatient settings. The current study aimed to sequence the genome of uropathogenic Escherichia coli strain CUI-B1 resourced from a woman having uncomplicated cystitis and pyelonephritis. Followed by deductive genomics towards potential drug targets using E. coli strain CUI-B1, strain O25b: H4-ST131, Proteus mirabilis strain HI4320, Klebsiella pneumoniae strain 1721, and Staphylococcus saprophyticus strain ATCC 15305 uropathogenic strains. Comparative genome analysis revealed that genes related to the survival of E. coli, P. mirabilis, K. pneumoniae, and S. saprophyticus, such as genes of metal-requiring proteins, defense-associated genes, and genes associated with general physiology, were found to be highly conserved in the genomes including strain CUI-B1. However, the genes responsible for virulence and drug resistance, mainly those that are involved in bacterial secretion, fimbriae, adherence, and colonization, were found in various genomic regions and varied from one species to another or within the same species. Based on the genome sequence, virulence, and antimicrobial-resistant gene dataset, the subtractive proteomics approach revealed 22 proteins mapped to the pathogen's unique pathways and among them, entB, clbH, chuV, and ybtS were supposed to be potential drug targets and the single drug could be utilized for all above-mentioned strains. These results may provide the foundation for the optimal target for future discovery of drugs for E. coli-, P. mirabilis-, K. pneumoniae-, and S. saprophyticus-based infections and could be investigated further to employ in personalized drug development.(AU)
Descriptores: infecciones urinarias/microbiología
virulencia
resistencia a medicamentos
Escherichia coli/genética
factores de virulencia
antibacterianos
-Bacteria/genética
Bacteria/metabolismo
microbiología
técnicas microbiológicas
Límites: seres humanos
Responsable: ES15.1 - BNCS


  3 / 790 IBECS  
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Id: 230263
Autor: Tang, Hai; Zhong, Zhi; Hou, Jingqing; You, Lijun; Zhao, Zhixin; Kwok, Lai-Yu; Bilige, Menghe.
Título: Metagenomic analysis revealed the potential of lactic acid bacteria in improving natural saline-alkali land / El análisis metagenómico reveló el potencial de las bacterias del ácido láctico para mejorar las tierras salino-álcalis naturales
Fuente: Int. microbiol;27(1):311-324, Feb. 2024. graf.
Idioma: en.
doi: 10.1007/s10123-023-00388-4.
Resumen: Management and improving saline-alkali land is necessary for sustainable agricultural development. We conducted a field experiment to investigate the effects of spraying lactic acid bacteria (LAB) on the cucumber and tomato plant soils. Three treatments were designed, including spraying of water, viable or sterilized LAB preparations to the soils of cucumber and tomato plants every 20 days. Spraying sterilized or viable LAB could reduce the soil pH, with a more obvious effect by using viable LAB, particularly after multiple applications. Metagenomic sequencing revealed that the soil microbiota in LAB-treated groups had higher alpha-diversity and more nitrogen-fixing bacteria compared with the water-treated groups. Both viable and sterilized LAB, but not water application, increased the complexity of the soil microbiota interactive network. The LAB-treated subgroups were enriched in some KEGG pathways compared with water or sterilized LAB subgroups, such as environmental information processingûrelated pathways in cucumber plant; and metabolism-related pathways in tomato plant, respectively. Redundancy analysis revealed association between some soil physico-chemical parameters (namely soil pH and total nitrogen) and bacterial biomarkers (namely Rhodocyclaceae, Pseudomonadaceae, Gemmatimonadaceae, and Nitrosomonadales). Our study demonstrated that LAB is a suitable strategy for decreasing soil pH and improving the microbial communities in saline-alkali land.(AU)
Descriptores: Bacteria/genética
microbiología del suelo
plantas
álcalis
Lactobacillales
metagenoma
-microbiología
técnicas microbiológicas
suelo
biotecnología/métodos
metagenómica
agua/metabolismo
Límites: seres humanos
Responsable: ES15.1 - BNCS


  4 / 790 IBECS  
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Texto Completo
Id: 230262
Autor: König, Patricia; Averhoff, Beate; Müller, Volker.
Título: K+ homeostasis is important for survival of Acinetobacter baumannii ATCC 19606 in the nosocomial environment / La homeostasis del K+ es importante para la supervivencia de Acinetobacter baumannii ATCC 19606 en el ambiente nosocomial
Fuente: Int. microbiol;27(1):303-310, Feb. 2024. graf.
Idioma: en.
doi: 10.1007/s10123-023-00389-3.
Resumen: Pathogenic bacteria have developed several mechanisms to thrive within the hostile environment of the human host, but it is often disregarded that their survival outside this niche is crucial for their successful transmission. Acinetobacter baumannii is very well adapted to both the human host and the hospital environment. The latter is facilitated by multifactorial mechanisms including its outstanding ability to survive on dry surfaces, its high metabolic diversity, and, of course, its remarkable osmotic resistance. As a first response to changing osmolarities, bacteria accumulate K+ in high amount to counterbalance the external ionic strength. Here, we addressed whether K+ uptake is involved in the challenges imposed by the harsh conditions outside its host and how K+ import influences the antibiotic resistance of A. baumannii. For this purpose, we used a strain lacking all major K+ importer ∆kup∆trk∆kdp. Survival of this mutant was strongly impaired under nutrient limitation in comparison to the wild type. Furthermore, we found that not only the resistance against copper but also against the disinfectant chlorhexidine was reduced in the triple mutant compared to the wild type. Finally, we revealed that the triple mutant is highly susceptible to a broad range of antibiotics and antimicrobial peptides. By studying mutants, in which the K+ transporter were deleted individually, we provide evidence that this effect is a consequence of the altered K+ uptake machinery. Conclusively, this study provides supporting information on the relevance of K+ homeostasis in the adaptation of A. baumannii to the nosocomial environment.(AU)
Descriptores: homeostasis
Acinetobacter baumannii/genética
antibacterianos/metabolismo
proteínas de transporte de membrana
Bacteria
virulencia
-microbiología
técnicas microbiológicas
antibacterianos/farmacología
Límites: seres humanos
Responsable: ES15.1 - BNCS


  5 / 790 IBECS  
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Id: 230261
Autor: Chakraborty, Sohini; Mondal, Sandhimita.
Título: Halotolerant Citrobacter sp. remediates salinity stress and promotes the growth of Vigna radiata (L) by secreting extracellular polymeric substances (EPS) and biofilm formation: a novel active cell for microbial desalination cell (MDC) / Citrobacter sp. halotolerante. remedia el estrés salino y promueve el crecimiento de Vigna radiata (L) mediante la secreción de sustancias poliméricas extracelulares (EPS) y la formación de biopelículas: una nueva célula activa para la célula microbiana de desalinización (MDC)
Fuente: Int. microbiol;27(1):291-301, Feb. 2024. ilus, graf.
Idioma: en.
doi: 10.1007/s10123-023-00386-6.
Resumen: To address soil salinization and its impact on crop production, microbial desalination cells (MDCs) offer a promising solution. These bioelectrochemical systems integrate desalination and wastewater treatment through microbial activity. A halotolerant beneficial bacterial strain called Citrobacter sp. strain KUT (CKUT) was isolated from India's salt desert Run of Kutch, Gujrat, highlighting its potential application in combating soil salinization. CKUT exhibits high salt tolerance and has the ability to produce extracellular polymeric substances (EPS) at a concentration of 0.04 mg/ml. It forms biofilm that enable it to withstand up to 10% NaCl concentration. Additionally, CKUT shows promise in remediating salinity levels, reducing it from 4.5 to 2.7 gL−1. These characteristics are driven by biofilm formation and EPS production. In an experiment where V. radiata L. seedlings were inoculated with CKUT, the treated plants exhibited enhanced chlorophyll content, growth, and overall plant characteristics compared to seedlings treated with sodium chloride (NaCl). These improvements included increased shoot length (150 mm), root length (40 mm), and biomass. This indicates that CKUT treatment has the potential to enhance the suitability of V. radiata and other crops for cultivation in saline lands, effectively addressing the issue of soil salinization. Furthermore, integrating CKUT into microbial desalination cells (MDCs) offers an opportunity for freshwater production from seawater, contributing to sustainable agriculture by promoting improved crop growth and increased yield in areas prone to salinity.(AU)
Descriptores: polifenoles
biopelículas
desalación
estrés por sales
Citrobacter
suelo/química
-microbiología
técnicas microbiológicas
microbiología del suelo
cloruro sódico
tolerancia a la sal
salinidad
Límites: seres humanos
Responsable: ES15.1 - BNCS


  6 / 790 IBECS  
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Texto Completo
Id: 230260
Autor: Gao, Wenhui; Li, Congcong; Wang, Fengtian; Yang, Yilin; Zhang, Lu; Wang, Zhongxue; Chen, Xi; Tan, Meixia; Cao, Guangxiang; Zong, Gongli.
Título: An efflux pump in genomic island GI-M202a mediates the transfer of polymyxin B resistance in Pandoraea pnomenusa M202 / Una bomba de eflujo en la isla genómica GI-M202a media la transferencia de resistencia a la polimixina B en Pandoraea pnomenusa M202
Fuente: Int. microbiol;27(1):277-290, Feb. 2024. ilus, graf.
Idioma: en.
doi: 10.1007/s10123-023-00384-8.
Resumen: Background: Polymyxin B is considered a last-line therapeutic option against multidrug-resistant gram-negative bacteria, especially in COVID-19 coinfections or other serious infections. However, the risk of antimicrobial resistance and its spread to the environment should be brought to the forefront. Methods: Pandoraea pnomenusa M202 was isolated under selection with 8 mg/L polymyxin B from hospital sewage and then was sequenced by the PacBio RS II and Illumina HiSeq 4000 platforms. Mating experiments were performed to evaluate the transfer of the major facilitator superfamily (MFS) transporter in genomic islands (GIs) to Escherichia coli 25DN. The recombinant E. coli strain Mrc-3 harboring MFS transporter encoding gene FKQ53_RS21695 was also constructed. The influence of efflux pump inhibitors (EPIs) on MICs was determined. The mechanism of polymyxin B excretion mediated by FKQ53_RS21695 was investigated by Discovery Studio 2.0 based on homology modeling. Results: The MIC of polymyxin B for the multidrug-resistant bacterial strain P. pnomenusa M202, isolated from hospital sewage, was 96 mg/L. GI-M202a, harboring an MFS transporter-encoding gene and conjugative transfer protein-encoding genes of the type IV secretion system, was identified in P. pnomenusa M202. The mating experiment between M202 and E. coli 25DN reflected the transferability of polymyxin B resistance via GI-M202a. EPI and heterogeneous expression assays also suggested that the MFS transporter gene FKQ53_RS21695 in GI-M202a was responsible for polymyxin B resistance. Molecular docking revealed that the polymyxin B fatty acyl group inserts into the hydrophobic region of the transmembrane core with Pi-alkyl and unfavorable bump interactions, and then polymyxin B rotates around Tyr43 to externally display the peptide group during the efflux process, accompanied by an inward-to-outward conformational change in the MFS transporter...(AU)
Descriptores: polimixina B
simulación de acoplamiento molecular
proteínas de transporte de membrana
islas genómicas
antibacterianos
Escherichia coli/genética
-microbiología
técnicas microbiológicas
aguas residuales
pruebas de sensibilidad microbiana
Límites: seres humanos
Responsable: ES15.1 - BNCS


  7 / 790 IBECS  
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Id: 230259
Autor: Niu, Xiaohong; Lu, Panpan; Huang, Linqing; Sun, Yan; Jin, Miaomiao; Liu, Jing; Li, Xing.
Título: The effect of metformin combined with liraglutide on gut microbiota of Chinese patients with type 2 diabetes / El efecto de la metformina combinada con liraglutida sobre la microbiota intestinal de pacientes chinos con diabetes tipo 2
Fuente: Int. microbiol;27(1):265-276, Feb. 2024. graf.
Idioma: en.
doi: 10.1007/s10123-023-00380-y.
Resumen: Background: Metformin (MET) is a first-line therapy for type-2 diabetes mellitus (T2DM). Liraglutide (LRG) is a glucagon-like peptide-1 receptor agonist used as a second-line therapy in combination with MET. Methods: We performed a longitudinal analysis comparing the gut microbiota of overweight and/or pre-diabetic participants (NCP group) with that of each following their progression to T2DM diagnosis (UNT group) using 16S ribosomal RNA gene sequencing of fecal bacteria samples. We also examined the effects of MET (MET group) and MET plus LRG (MET+LRG group) on the gut microbiota of these participants following 60 days of anti-diabetic drug therapy in two parallel treatment arms. Results: In the UNT group, the relative abundances of Paraprevotella (P = 0.002) and Megamonas (P = 0.029) were greater, and that of Lachnospira (P = 0.003) was lower, compared with the NCP group. In the MET group, the relative abundance of Bacteroides (P = 0.039) was greater, and those of Paraprevotella (P = 0.018), Blautia (P = 0.001), and Faecalibacterium (P = 0.005) were lower, compared with the UNT group. In the MET+LRG group, the relative abundances of Blautia (P = 0.005) and Dialister (P = 0.045) were significantly lower than in the UNT group. The relative abundance of Megasphaera in the MET group was significantly greater than in the MET+LRG group (P = 0.041). Conclusions: Treatment with MET and MET+LRG results in significant alterations in gut microbiota, compared with the profiles of patients at the time of T2DM diagnosis. These alterations differed significantly between the MET and MET+LRG groups, which suggests that LRG exerted an additive effect on the composition of gut microbiota.(AU)
Descriptores: diabetes mellitus tipo II
metformina
microbiota intestinal
liraglutida/farmacología
ARN ribosómico 16S
-microbiología
técnicas microbiológicas
China
liraglutida/uso terapéutico
Límites: seres humanos
Responsable: ES15.1 - BNCS


  8 / 790 IBECS  
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Id: 230258
Autor: Juárez-Cepeda, Jacqueline; Valenzuela, Olivia; Garibay-Valdez, Estefanía; Velazquez, Carlos; Garibay-Escobar, Adriana.
Título: Gene expression during the development of Mycobacterium smegmatis biofilms on hydroxyapatite surfaces / Expresión génica durante el desarrollo de biopelículas de Mycobacterium smegmatis en superficies de hidroxiapatita
Fuente: Int. microbiol;27(1):257-263, Feb. 2024. graf.
Idioma: en.
doi: 10.1007/s10123-023-00385-7.
Resumen: Bacterial biofilms are a consortium of bacteria that are strongly bound to each other and the surface on which they developed irreversibly. Bacteria can survive adverse environmental conditions and undergo changes when transitioning from a planktonic form to community cells. The process of mycobacteria adhesion is complex, involving characteristics and properties of bacteria, surfaces, and environmental factors; therefore, the formation of different biofilms is possible. Cell wall-, lipid-, and lipid transporter-related genes (glycopeptidolipids, GroEL1, protein kinase) are important in mycobacterial biofilm development. We investigated gene expression during in vitro development of Mycobacterium smegmatis biofilms on a hydroxyapatite (HAP) surface. Biofilm formation by M. smegmatis cells was induced for 1, 2, 3, and 5 days on the HAP surface. Mycobacteria on polystyrene generated an airûliquid interface biofilm, and on the fifth day, it increased by 35% in the presence of HAP. Six genes with key roles in biofilm formation were analyzed by real-time RT‒qPCR during the biofilm formation of M. smegmatis on both abiotic surfaces. The expression of groEL1, lsr2, mmpL11, mps, pknF, and rpoZ genes during biofilm formation on the HAP surface did not exhibit significant changes compared to the polystyrene surface. These genes involved in biofilm formation are not affected by HAP.(AU)
Descriptores: durapatita
Mycobacterium smegmatis
biopelículas
proteínas bacterianas/genética
expresión génica
hidroxiapatitas/metabolismo
-microbiología
técnicas microbiológicas
proteínas bacterianas/metabolismo
lípidos
poliestirenos/metabolismo
Límites: seres humanos
Responsable: ES15.1 - BNCS


  9 / 790 IBECS  
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Id: 230257
Autor: Bachtarzi, Nadia; Gomri, Mohamed Amine; Meradji, Meriem; Gil-Cardoso, Katherine; Ortega, Nàdia; Chomiciute, Gertruda; Bas, Josep Maria Del; López, Quiro; Martínez, Vanesa; Kharroub, Karima.
Título: In vitro assessment of biofunctional properties of Lactiplantibacillus plantarum strain Jb21-11 and the characterization of its exopolysaccharide / Evaluación in vitro de las propiedades biofuncionales de Lactiplantibacillus plantarum cepa Jb21-11 y caracterización de su exopolisacárido
Fuente: Int. microbiol;27(1):239-256, Feb. 2024. graf.
Idioma: en.
doi: 10.1007/s10123-023-00387-5.
Resumen: The microbiota of traditional food provides a rich reservoir of biodiversity to find new strains with interesting features for novel functional food formulation. Therefore, this study aimed to investigate the biofunctional potential of the lactic acid bacteria (LAB) strain Jb21-11 isolated from Jben, a traditional Algerian fresh cheese. This isolate was selected out of a collection of 154 LAB based on its exopolysaccharide (EPS) phenotype and was preliminarily identified by polyphasic characterization as Lactiplantibacillus plantarum (previously known as Lactobacillus plantarum) and its biofunctional properties were then assessed in vitro. The tested strain demonstrated good resistance to gastric juice, acidity around pH 2, and 2% (v/v) bile salts, which are important characteristics for potential biofunctional LAB candidates. It also showed a good production of ropy EPS with 674 mg/L on MRS medium. However, this ability appears to compromise the adhesion of the strain to Caco-2 cells (less than 1%), which according to our results, seems not to be related to autoaggregation and hydrophobicity (44.88 ± 0.028% and 16.59 ± 0.012%). Furthermore, promising antimicrobial activity against three pathogenic bacteria (Escherichia coli, Staphylococcus aureus, and Salmonella) was detected probably due to antimicrobial metabolites excreted during fermentation process into the medium. Moreover, the strain L. plantarum Jb21-11 displayed a therapeutic functionality with both anti-inflammatory and immunomodulatory action using RAW 264.7 cells. The chemical features of the novel ropy Jb21-11-EPS were also investigated revealing the presence of three monosaccharides, namely, mannose, galactose, and glucose, with a molar ratio of 5.42:1.00:4.52 linked together by α- and β-glycosidic bonds, presenting a relatively high molecular weight of 1.08 × 105 Da of interest for a texturing potential. Therefore, the new producing EPS strain Jb21-11 is a promising candidate for use as an adjunct culture for improving the texture of functional food.(AU)
Descriptores: alimentos
microbiología de los alimentos
microbiota
probióticos
Lactobacillus plantarum
Lactobacillales
-microbiología
técnicas microbiológicas
Escherichia coli
antiinfecciosos
células CACO-2
ácido láctico
Límites: seres humanos
Responsable: ES15.1 - BNCS


  10 / 790 IBECS  
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Id: 230256
Autor: Angal, Ashvini; Shidture, Shubham; Syed, Jaserah; Tiwari, Deepika Pandey; Dubey, Ashok Kumar; Bhaduri, Anirban; Pujari, Radha.
Título: In vitro adhesion and anti-inflammatory properties of Limosilactobacillus fermentum FS-10 isolated from infant fecal sample / Adhesión in vitro y propiedades antiinflamatorias de Limosilactobacillus fermentum FS-10 aislado de muestra fecal infantil
Fuente: Int. microbiol;27(1):227-238, Feb. 2024. graf, tab.
Idioma: en.
doi: 10.1007/s10123-023-00383-9.
Resumen: In this study, seven strains of Limosilactobacillus fermentum were isolated from an infant fecal sample and characterized using in vitro studies. Lactobacillus rhamnosus GG was used as a comparison because it is a well-documented commercial probiotic. The isolates were tested for attributes such as acid and phenol tolerance, bile salt hydrolase (BSH) activity, and antibiotic sensitivity. One isolate, L. fermentum FS-10, displayed enhanced cell surface hydrophobicity (> 85%) and mucin adhesion. Mucin-binding helps colonization in the gut. The immunomodulatory property of L. fermentum FS-10 was evaluated by determining the modulation of pro- and anti-inflammatory factors such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-10, and nitric oxide (NO) in human acute monocytic leukemia (THP-1) cells under inflammatory conditions induced by lipopolysaccharide (LPS). L. fermentum FS-10 potently downregulated the expression of TNF-α and nitric oxide and upregulated IL-10 levels, indicating an anti-inflammatory response. Safety assessment of the strain revealed the absence of genes for virulence factors, toxin production, and antibiotic resistance, potentiating application as a probiotic strain.(AU)
Descriptores: coliformes
heces/microbiología
LIMOSILACTOBACILLUS FERMENTUM
probióticos
antiinflamatorios
factor de necrosis tumoral alfa
-microbiología
técnicas microbiológicas
mucinas
óxido nítrico
Límites: seres humanos
masculino
femenino
niño
Responsable: ES15.1 - BNCS



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