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Búsqueda : "1139-6709" [ISSN]
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Id: 2
Autor: Ortega, Leonel Maximiliano; Moure, María Candela; González, Esteban Manuel; Alconada, Teresa María.
Título: Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of Fusarium graminearum
Fuente: Int. microbiol;22(2):289-296, jun. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-018-00048-y.
Resumen: Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grain infection with Fusarium graminearum produce changes in the glutenin content that affect the baking properties. This work analyzes the high-molecular-weight glutenin changes from wheat flour with different degrees of F. graminearum infection at field, since these proteins are determinant for the quality properties of flour. Wheat cultivars-on field trials-infected with F. graminearum isolates of diverse aggressiveness showed severity values between 9.1 and 42.58% and thousand kernel weight values between 28.12 and 32.33 g. Negative correlations between severity and protein content and positive correlations between yield and protein content were observed, employing reversed-phase high-performance liquid chromatography and polyacrylamide gel electrophoresis. Furthermore, the protein signal changes were in agreement for both methodological approaches. Also, the degree of disease observed and the protein changes on infected wheat cultivars varied in relation with the aggressiveness of the isolate responsible for the infection. The principal component analysis showed a close arrangement among protein values obtained by HPLC. For each cultivar, two principal components were obtained, which explained 80.85%, 88.48%, and 93.33% of the total variance (cultivars Sy200, AGP Fast, and Klein Tigre respectively). To our knowledge, the approaches employed for the analysis of protein changes according to the degree of disease, as well as the thorough statistical analysis, are novel for the study of Fusarium Head Blight

No disponible
Descriptores: Fusarium/metabolismo
enfermedades de las plantas/microbiología
proteínas de plantas/análisis
Fusarium/crecimiento & desarrollo
Responsable: BNCS

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Id: 185070
Autor: Gozari, Mohsen; Zaheri, Ahmad; Jahromi, Saeid Tamadoni; Gozari, Majid; Karimzadeh, Ramin.
Título: Screening and characterization of marine actinomycetes from the northern Oman Sea sediments for cytotoxic and antimicrobial activity
Fuente: Int. microbiol;22(4):521-530, dic. 2019. mapas, graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00083-3.
Resumen: A total of 168 actinomycete colonies were isolated from 14 sediment samples of the northern parts of the Oman Sea and were screened for cytotoxic and antimicrobial activity. Among four media and two treatments, the glucose arginine agar medium (18%) and heat treatment (28.3%) showed maximum isolation rate of actinomycetes. Preliminary characterization revealed that the members of Streptomycetaceae were widely distributed (66%) in the most of the sampling stations followed by Micromonosporaceae (14%), Nocardiaceae (6%), and Pseudonocardiaceae (4%), respectively. Approximately, 23.8% of the isolates inhibited the growth of at least one of the microbial test strains, while the majority of them belonged to the Streptomycetaceae family. Minimum inhibitory concentrations of the ethyl acetate culture extracts of the five most putative isolates varied from 64 μg/mL against Micrococcus luteus and Candida albicans to 1 mg/mL against Aspergillus niger. These extracts showed significant cytotoxic activity at18.74-193.5 μg/mL on the human breast (MCF7), colon (HCT 116), and liver (HepG2) tumor cell lines while exhibited less or no cytotoxicity on the normal cell line (HUVEC). Interestingly, IFSRI 193 extract selectively inhibited the growth of HCT 116 cell line and gram-positive bacteria. 16S rRNA gene sequencing revealed that the potent isolates have 97 to 99% similarity with S. chartreusis, S. cacaoi, S. sampsonii, S. qinglanensis, and S. diastaticus. These results suggested that the five Streptomyces strains could be considered candidates for discovering the antitumor antibiotics

No disponible
Descriptores: infecciones por Actinomycetales/microbiología
sedimentos marinos (salud ambiental)/análisis
Streptomycetaceae/aislamiento & purificación
-Micromonosporaceae/aislamiento & purificación
Nocardiaceae/aislamiento & purificación
ARN ribosómico 16S/aislamiento & purificación
células HCT116/metabolismo
células HCT116/microbiología
Responsable: BNCS

  3 / 562 IBECS  
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Id: 185069
Autor: Orem, JC; Silva, WMC; Raiol, T; Magalhães, MI; Martins, PH; Cavalcante, DA; Kruger, RH; Brigido, MM; De-Souza, MT.
Título: Phylogenetic diversity of aerobic spore-forming Bacillalles isolated from Brazilian soils
Fuente: Int. microbiol;22(4):511-520, dic. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00080-6.
Resumen: The phylum Firmicutes comprises seven classes where most species are either aerobic or anaerobic endospore former. Inside Firmicutes, species allocated in the genus Bacillus and related genera are collectively named aerobic endospore-forming bacteria (AEFB), and the soil is their major reservoir. AEFB have great importance in health, agriculture, and biotechnology although the more studied species are Bacillus subtilis and the human pathogens Bacillus cereus and Bacillus anthracis. AEFB have great importance in health, agriculture, and biotechnology; although the knowledge about these organisms is based on few species, notably, Bacillus subtilis, Bacillus cereus, and Bacillus anthracis. In this work, we generated partial 16S rRNA gene sequences of both strands of 192 AEFB strains isolated from soils of Distrito Federal, Brazil (SDF strains). The resulting consensus sequences were used to obtain taxonomic assignment and establish the phylogenetic relationships among these strains. Through this approach, we could observe that classified SDF strains were distributed among genera Bacillus (169 strains; 88.02%), Paenibacillus (11; 5.73%), Lysinibacillus (6; 3.13%), Brevibacillus (4; 2.08%), Terribacillus (1; 0.52%), and Rummeliibacillus (1; 0.52%). Phylogenetic trees revealed these 192 SDF strains can be segregated into eight groups spanning families Bacillaceae and Paenibacillaceae belonging to the order Bacillales. To expand the knowledge about the diversity of these SDF strains, further studies regarding characterization with different methodologies are underway

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Descriptores: contaminantes del suelo/análisis
análisis del suelo
esporas bacterianas/aislamiento & purificación
recuento de colonias microbianas
ARN ribosómico 16S/genética
bacterias anaerobias/aislamiento & purificación
Bacillales/aislamiento & purificación
esporas fúngicas/genética
perfiles de expresión génica/métodos
Responsable: BNCS

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Id: 185068
Autor: Siddique, Mahbubul Pratik; Jang, Won Je; Lee, Jong Min; Hasan, Md Tawheed; Kim, Chang-Hoon; Kong, In-Soo.
Título: Detection of Vibrio anguillarum and Vibrio alginolyticus by Singleplex and Duplex Loop-Mediated Isothermal Amplification (LAMP) Assays Targeted to groEL and fklB Genes
Fuente: Int. microbiol;22(4):501-509, dic. 2019. ilus, graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00079-z.
Resumen: Singleplex and duplex loop-mediated isothermal amplification (LAMP) assays were developed for detecting Vibrio anguillarum, a major bacterial pathogen of fish, and Vibrio alginolyticus, a pathogen of fish and humans, separately and simultaneously from contaminated seawater by targeting the groEL gene of V. anguillarum, which encodes a molecular chaperone protein, and the fklB gene of V. alginolyticus, which encodes a 22 kilodalton (kDa) peptidyl prolyl isomerase. The optimal reaction conditions to produce consistent results were 65ºC for 30 min, 63ºC for 30 min, and 63ºC for 40 min for the groEL (singleplex for V. anguillarum), fklB (singleplex for V. alginolyticus), and groEL + flkB (duplex) LAMP assays, respectively, analyzed via visual detection methods (use of calcein, and SYBR Green I) and agarose gel electrophoresis. The assays were found to be species-specific, as closely related Vibrio spp. were not detected. The limits of detection (LoDs) of the LAMP assays for DNA template from pure culture and artificially contaminated seawater were 10 and 14 fg (groEL assay; for V. anguillarum), 12.5 and 17 fg (fklB assay; for V. alginolyticus), and 50 and 70 fg (duplex assay) per reaction, respectively, which were much better than the LoDs of conventional polymerase chain reaction (PCR). Singleplex and duplex LAMP assays were found to be rapid, species-specific, and sensitive for the detection of V. anguillarum and V. alginolyticus and are applicable to laboratory and field diagnostics

No disponible
Descriptores: Vibrio alginolyticus/aislamiento & purificación
técnicas de amplificación de ácidos nucleicos/métodos
-Vibrio/crecimiento & desarrollo
agua de mar/análisis
agua de mar/microbiología
reacción en cadena de la polimerasa
Límites: seres humanos
Responsable: BNCS

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Id: 185067
Autor: Wang, Wen; Wang, Aizhen; Yang, Yongsheng; Wang, Fang; Liu, Yingbao; Zhang, Yuhui; Sharshov, Kirill; Gui, Linsheng.
Título: Composition, diversity and function of gastrointestinal microbiota in wild red-billed choughs (Pyrrhocorax pyrrhocorax)
Fuente: Int. microbiol;22(4):491-500, dic. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00076-2.
Resumen: Hitherto, virtually nothing is known about the microbial communities related to the bird species in the family Corvidae. To fill this gap, the present study was conducted to provide a baseline description of the gut microbiota of wild red-billed choughs (Pyrrhocorax pyrrhocorax). In this study, microbiota from four gastrointestinal locations (oropharynx, gizzard, small intestine, and large intestine) of three wild red-billed choughs were analyzed using the Illumina MiSeq sequencing platform by targeting the V4-V5 regions of the 16S rRNA genes. The gut microbiota of the red-billed choughs were dominated by the phylum Firmicutes (59.56%), followed by Proteobacteria (16.56%), Bacteroidetes (13.86%), and Actinobacteria (7.03%), which were commonly detected in avian gut ecosystems. Genus-level compositions were found to be largely dominated by Lactobacillus (18.21%), Weissella (12.37%), Erysipelatoclostridium (6.94%), Bacteroides (6.63%), Escherichia-Shigella (5.15%), Leuconostoc (4.60%), Proteus (3.33%), Carnobacterium (2.71%), Lactococcus (1.69%), and Enterococcus (1.63%). The overall intestinal microbiota was enriched with functions related to ATP-binding cassette (ABC) transporters, DNA repair and recombination proteins, purine metabolism, ribosome, transcription factors, pyrimidine metabolism, peptidases, and two-component system. In terms of four different gastrointestinal locations, hierarchical clustering analysis and principal coordinate analysis showed that microbial communities of the oropharynx, gizzard, small intestine, and large intestine formed four separated clusters. A total of 825 OTUs and 382 genera were detected in all four gastrointestinal locations, which were considered as the major microbes in the intestines of red-billed choughs. Coexistence of lactic acid bacteria and potential pathogens in the gut environments of red-billed choughs required further investigations

No disponible
Descriptores: microbiota intestinal/genética
enfermedades de las aves/microbiología
ARN ribosómico 16S/aislamiento & purificación
-ARN ribosómico 16S/genética
Firmicutes/aislamiento & purificación
Proteobacteria/aislamiento & purificación
Bacteroidetes/aislamiento & purificación
Lactobacillus/aislamiento & purificación
Weissella/aislamiento & purificación
Límites: animales
Responsable: BNCS

  6 / 562 IBECS  
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Id: 185066
Autor: El-Bahar, Helmy Mohamed; Ali, Nadia Gabr; Aboyadak, Ibrahim Mohamed; Khalil, Samy Abd El Salam; Ibrahim, Madiha Salah.
Título: Virulence genes contributing to Aeromonas hydrophila pathogenicity in Oreochromis niloticus
Fuente: Int. microbiol;22(4):479-490, dic. 2019. ilus, tab.
Idioma: en.
doi: 10.1007/s10123-019-00075-3.
Resumen: Bacterial diseases are the main cause of high economic loss in aquaculture, particularly gram-negative bacteria. This study was conducted for the isolation and identification of Aeromonas and Pseudomonas spp. from diseased fish. Twenty-two Aeromonas and sixteen Pseudomonas isolates were recovered from diseased Nile tilapia (Oreochromis niloticus) raised in eight earthen ponds in Elhox, Metoubes, Kafrelsheikh, Egypt. The recovered isolates were further identified using PCR as 22 Aeromonas hydrophila, 11 Pseudomonas aeruginosa, and 5 Pseudomonas fluorescens isolates. The 22 A. hydrophila isolates were screened for the presence of four virulence genes. Sixteen of the isolates (72.72%) were positive for the aerolysin gene (aer); 4 (18.18%) harbored the cytotoxic enterotoxin gene (act); and 2 (9.09%) carried the hemolysin A gene (hylA) while the cytotonic heat-stable enterotoxin gene (ast) was absent from all the tested isolates. The pathogenicity test indicated the direct relationship between the mortality percentage and the genotype of the tested A. hydrophila isolates as the mortality rates were 63.3 and 73.3% for isolates with two virulence genes (aer+ & act+, and aer+ and hylA+, respectively), followed by 40, 53.3, and 56.6% for isolates with only one virulence gene (hylA, act, and aer, respectively) and 20% for isolates lacking virulence genes. Based on the sensitivity test, the multi-antibiotic resistance profiles were as follows: 90.9% of the A. hydrophila isolates were sensitive to florfenicol and doxycycline; then 68.18% were susceptible to oxytetracycline, norfloxacin, and ciprofloxacin; and 63.63% were susceptible to sulfamethoxazole-trimethoprim, while only 27.27 and 4.5% were sensitive to erythromycin and cephradine, respectively, and all the isolates were resistant to amoxicillin and ampicillin

No disponible
Descriptores: virulencia/genética
Aeromonas hydrophila/patogenicidad
-Aeromonas hydrophila/genética
Aeromonas hydrophila/aislamiento & purificación
norfloxacino/aislamiento & purificación
pruebas de sensibilidad microbiana/métodos
reacción en cadena de la polimerasa
Límites: animales
Responsable: BNCS

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Id: 185065
Autor: Haro-Cruz, María J de; Guadarrama-Macedo, Sandra I; López-Hurtado, Marcela; Escobedo-Guerra, Marcos R; Guerra-Infante, Fernando M.
Título: Obtaining an ELISA test based on a recombinant protein of Chlamydia trachomatis
Fuente: Int. microbiol;22(4):471-478, dic. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00074-4.
Resumen: Chlamydia trachomatis is considered as a public health problem due to its high prevalence and increased rates of gynecological disorders. The major outer membrane protein (MOMP) of this bacterium is the most abundant protein in its membrane and has been evaluated not only as a vaccine development candidate but also is used in many diagnostic tests. The MOMP weighs 69 kDa and contains four variable segments (VS 1-4) separated by constant regions. Several research groups have developed recombinant single-variable segments of MOMP expressed in Escherichia coli cytoplasm. But, all variable segments have been used minimally for the diagnosis of a chlamydial infection. In this experiment, the authors obtained the recombinant MOMP of C. trachomatis (rMOMP) in E. coli rMOMP and extracted, purified, and partially characterized it. This was later used to identify anti-Chlamydia trachomatis antibodies in sera of infertile patients by immunodetection assays, enzyme-linked immunosorbent assay (ELISA), and indirect immunofluorescence tests. The ELISA test showed high sensitivity and low specificity of 100 and 58.3%, respectively. The above results obtained were linked to the cross-reactivity of antibodies against C. pneumoniae or C. psittaci. Hence, an evaluation was performed to obtain an optimized test for the diagnosis of C. trachomatis infection

No disponible
Descriptores: antígenos bacterianos/análisis
Chlamydia trachomatis/aislamiento & purificación
infecciones por Chlamydia/diagnóstico
enzimoinmunoanálisis por adsorción/métodos
proteínas recombinantes/análisis
proteínas de la membrana externa bacteriana/metabolismo
-Chlamydia trachomatis/genética
infecciones por Chlamydia/microbiología
proteínas recombinantes/genética
Responsable: BNCS

  8 / 562 IBECS  
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Id: 185064
Autor: Schäfer, Lukas; Meinert-Berning, Christina; Wübbeler, Jan Hendrik; Steinbüchel, Alexander.
Título: A tripartite tricarboxylate transporter (MIM_c39170-MIM_c39210) of Advenella mimigardefordensis DPN7T is involved in citrate uptake
Fuente: Int. microbiol;22(4):461-470, dic. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00073-5.
Resumen: To date, tripartite tricarboxylate transport (TTT) systems are not well characterized in most organisms. To investigate which carbon sources are transported by the TTT system of A. mimigardefordensis DPN7T, single deletion mutants were generated lacking either completely both sets of genes encoding for these transport systems tctABCDE1 and tctABDE2 in the organism or the two genes encoding for the regulatory components of the third chosen TTT system, tctDE3. Deletion of tctABCDE1 (MIM_c39170-MIM_c39210) in Advenella mimigardefordensis strain DPN7T led to inhibition of growth of the cells with citrate indicating that TctABCDE1 is the transport system for the uptake of citrate. Because of the negative phenotype, it was concluded that this deletion cannot be substituted by other transporters encoded in the genome of strain DPN7T. A triple deletion mutant of A. mimigardefordensis lacking both complete TTT transport systems and the regulatory components of the third chosen system (ΔTctABCDE1 ΔTctABDE2 ΔTctDE3) showed a leaky growth with alpha-ketoglutarate in comparison with the wild type. The other investigated TTT (TctABDE3, MIM_c17190-MIM_c17220) is most probably involved in the transport of alpha-ketoglutarate. Additionally, thermoshift assays with TctC1 (MIM_c39190) showed a significant shift in the melting temperature of the protein in the presence of citrate whereas no shift occurred with alpha-ketoglutarate. A dissociation constant Kd for citrate of 41.7 μM was determined. Furthermore, alternative alpha-ketoglutarate transport was investigated via in silico analysis

No disponible
Descriptores: ácidos tricarboxílicos/metabolismo
succinato-CoA ligasas/metabolismo
-transportadores de ácidos dicarboxílicos/genética
ácidos dicarboxílicos/metabolismo
espectrometría de masas/métodos
ciclo del ácido cítrico
succinato-CoA ligasas/genética
Responsable: BNCS

  9 / 562 IBECS  
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Id: 185063
Autor: Kuncharoen, Nattakorn; Fukasawa, Wataru; Iwatsuki, Masato; Mori, Mihoko; Shiomi, Kazuro; Tanasupawat, Somboon.
Título: Characterisation of Two Polyketides from Streptomyces sp. SKH1-2 Isolated from Roots of Musa (ABB) cv. 'Kluai Sao Kratuep Ho'
Fuente: Int. microbiol;22(4):451-459, dic. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00071-7.
Resumen: An endophytic actinomycete strain SKH1-2 isolated from Musa (ABB) cv. 'Kluai Sao Kratuep Ho' collected in Suphan Buri province (14º 54′ 22.5″ N/100º 04′ 50″ E), Thailand, was identified as Streptomyces pseudovenezuelae based on phenotypic and chemotaxonomic characteristics, and 16S rRNA sequence analyses. A chemical investigation led to the isolation of two polyketide molecules from the n-butanol crude extract of the strain SKH1-2 culture broth. The compounds were purified using various chromatographic techniques and identified using spectroscopic methods compared with earlier published data. Compound 1, chartreusin, is known as an anti-Gram (+) bacterial compound and was active against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341 and Staphylococcus aureus ATCC 6538p with MIC values of 3.1, 1.6 and 12.5 μg/mL, respectively. Compound 2, lumichrome, did not show activity against all tested microbes. To our knowledge, this is the first report of chartreusin and lumichrome isolated from S. pseudovenezuelae. Taken together, it could be proved that Thai plant species are valuable reservoirs of interesting endophytic actinomycetes producing several interesting biologically active compounds

No disponible
Descriptores: policétidos/aislamiento & purificación
Streptomyces/aislamiento & purificación
ARN ribosómico 16S/aislamiento & purificación
Staphylococcus aureus/aislamiento & purificación
ARN ribosómico 16S/química
análisis espectral
Responsable: BNCS

  10 / 562 IBECS  
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Id: 185062
Autor: Nguyen, Van Duy; Nguyen, Thanh Tra; Pham, Thu Thuy; Packianather, Michael; Le, Chi Hieu.
Título: Molecular screening and genetic diversity analysis of anticancer Azurin-encoding and Azurin-like genes in human gut microbiome deduced through cultivation-dependent and cultivation-independent studies
Fuente: Int. microbiol;22(4):437-449, dic. 2019. graf, tab.
Idioma: en.
doi: 10.1007/s10123-019-00070-8.
Resumen: Azurin, a bacteriocin produced by a human gut bacterium Pseudomonas aeruginosa, can reveal selectively cytotoxic and induce apoptosis in cancer cells. After overcoming two phase I trials, a functional region of Azurin called p28 has been approved as a drug for the treatment of brain tumor glioma by FDA. The present study aims to improve a screening procedure and assess genetic diversity of Azurin genes in P. aeruginosa and Azurin-like genes in the gut microbiome of a specific population in Vietnam and global populations. Firstly, both cultivation-dependent and cultivation-independent techniques based on genomic and metagenomic DNAs extracted from fecal samples of the healthy specific population were performed and optimized to detect Azurin genes. Secondly, the Azurin gene sequences were analyzed and compared with global populations by using bioinformatics tools. Finally, the screening procedure improved from the first step was applied for screening Azurin-like genes, followed by the protein synthesis and NCI in vitro screening for anticancer activity. As a result, this study has successfully optimized the annealing temperatures to amplify DNAs for screening Azurin genes and applying to Azurin-like genes from human gut microbiota. The novelty of this study is the first of its kind to classify Azurin genes into five different genotypes at a global scale and confirm the potential anticancer activity of three Azurin-like synthetic proteins (Cnazu1, Dlazu11, and Ruazu12). The results contribute to the procedure development applied for screening anticancer proteins from human microbiome and a comprehensive understanding of their therapeutic response at a genetic level

No disponible
Descriptores: azurina/genética
técnicas in vitro/métodos
variación genética/efectos de los fármacos
microbiota intestinal/genética
-azurina/uso terapéutico
microbiota intestinal/efectos de los fármacos
biología computacional/métodos
Responsable: BNCS

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